goat anti–munc13-4 (Absolute Biotech Inc)
90
Structured Review
Absolute Biotech Inc
goat anti–munc13-4
Goat Anti–Munc13 4, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti%E2%80%93munc13-4/goat+anti+munc13+4/pmc04418747-62-56-57
Average 90 stars, based on 1 article reviews
Goat Anti–Munc13 4, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti%E2%80%93munc13-4/goat+anti+munc13+4/pmc04418747-62-56-57
Average 90 stars, based on 1 article reviews
goat anti–munc13-4 - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding"
Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding
Journal: The Journal of Allergy and Clinical Immunology
doi: 10.1016/j.jaci.2014.08.039
Figure Legend Snippet: CTLs from patients UPN396 and UPN324 show severely reduced ability to kill target cells. A, Percentage killing of P815 target cells by means of redirected lysis with anti-CD3 mediated by CTLs from a healthy donor ( HD ; triangles ), patient UPN324 (squares) , and patient UPN396 (circles) after 4 hours. Each data point was run in triplicates. The image is representative of 3 independent experiments. B, Immunoblots of Rab27a and Munc13-4 in CTLs from a healthy donor (HD) and patients UPN324 and UPN396. Proteins from lysates were separated by means of SDS-PAGE, transferred onto nitrocellulose, and probed with protein-specific antibodies against Rab27a, Munc13-4, and actin. Images are representative of 3 independent experiments.
Techniques Used: Lysis, Western Blot, SDS Page
Figure Legend Snippet: Effect of mutations in Rab27a on binding to Mlph and Munc13-4. Immunoblots showing coimmunoprecipitations are shown. A, Munc13-4 with Rab27a ( lanes 1 and 2 ), Rab27a-S163R ( lanes 3 and 4 ), and Rab27a-R141lfs ( lanes 5 and 6 ). Images are representative of 3 independent experiments. B, Rab27a ( lanes 1 and 2 ), Rab27a-S163R ( lanes 3 and 4 ), and Rab27a-R141lfs ( lanes 5 and 6 ) with Mlph. Membranes were probed with specific antibodies against Rab27a (top) or Mlph (bottom) . C and D, As above but with Rab27A A76V and Y159C. Proteins were immunoprecipitated with anti-Munc13-4 or Mlph and probed for Rab27a, Munc13-4, and Mlph.
Techniques Used: Binding Assay, Western Blot, Immunoprecipitation
Related Articles
Produced:Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding Article Snippet: Lysates were precleared with 100 μL of Protein G Bead slurry (GE Healthcare, Fairfield, Conn) with rotating at 4°C for 30 minutes and then split into 3 samples: no antibody, rabbit anti-Rab27a (produced in rabbits immunized with human Rab27a-GST and screened against lysates from healthy donor and Rab27A-deficient human and mouse cell lines), and either goat Lysis:Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding Article Snippet: Lysates were precleared with 100 μL of Protein G Bead slurry (GE Healthcare, Fairfield, Conn) with rotating at 4°C for 30 minutes and then split into 3 samples: no antibody, rabbit anti-Rab27a (produced in rabbits immunized with human Rab27a-GST and screened against lysates from healthy donor and Rab27A-deficient human and mouse cell lines), and either goat Western Blot:Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding Article Snippet: Lysates were precleared with 100 μL of Protein G Bead slurry (GE Healthcare, Fairfield, Conn) with rotating at 4°C for 30 minutes and then split into 3 samples: no antibody, rabbit anti-Rab27a (produced in rabbits immunized with human Rab27a-GST and screened against lysates from healthy donor and Rab27A-deficient human and mouse cell lines), and either goat SDS Page:Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding Article Snippet: Lysates were precleared with 100 μL of Protein G Bead slurry (GE Healthcare, Fairfield, Conn) with rotating at 4°C for 30 minutes and then split into 3 samples: no antibody, rabbit anti-Rab27a (produced in rabbits immunized with human Rab27a-GST and screened against lysates from healthy donor and Rab27A-deficient human and mouse cell lines), and either goat Binding Assay:Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding Article Snippet: Lysates were precleared with 100 μL of Protein G Bead slurry (GE Healthcare, Fairfield, Conn) with rotating at 4°C for 30 minutes and then split into 3 samples: no antibody, rabbit anti-Rab27a (produced in rabbits immunized with human Rab27a-GST and screened against lysates from healthy donor and Rab27A-deficient human and mouse cell lines), and either goat Immunoprecipitation:Article Title: Patients with Griscelli syndrome and normal pigmentation identify RAB27A mutations that selectively disrupt MUNC13-4 binding Article Snippet: Lysates were precleared with 100 μL of Protein G Bead slurry (GE Healthcare, Fairfield, Conn) with rotating at 4°C for 30 minutes and then split into 3 samples: no antibody, rabbit anti-Rab27a (produced in rabbits immunized with human Rab27a-GST and screened against lysates from healthy donor and Rab27A-deficient human and mouse cell lines), and either goat |